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HPV infection in HIVinfected men who have sex with men and correlation with HHV8 shedding

Abstract number: O503

Parisi S., Boldrin C., Scaggiante R., Cruciani M., Andreis S., Barelli A., Pagni S., Dal Bello F., Palù G.

Objectives: To evaluate HPV genotypes circulation and persistence over time in different sampling sites among HIV(+) men who have sex with men (MSM) (pts) and correlations with HHV8 oral shedding.

Methods: Samples obtained from rectal mucosa- (RS), pharyngeal-swabs (PhS) and saliva of 118 pts from December 2007 to December 2008 were studied. After 6 mo, samples were collected again. Amplification products were sequenced by in-house method using ABI PRISM 3130 XL. HHV8 oral shedding was tested by Real-Time in-house method.

Results: HPV was found in 97 (82%) of RS: 9 pts had multiple HPV-genotypes, and 82 pts a single genotype or an identified type with a co-infection with other not-identified types [32 (39%) High-Risk genotypes (HR) – 11 HPV-16, 1 HPV-18, 2 HPV-33, 1 HPV-53, 6 HPV-58, 11 HPV-66-; 42 Low Risk genotypes (LR) – 12 HPV-6, 6 HPV-11, 1 HPV-40, 7 HPV-61, 5 HPV-70, 2 HPV-72, 4 HPV-81, 5 HPV-83, and 8 other types]. 2 pts had amplification products not further analysable, 4 typing are ongoing and 21 pts were negative.

After 6 mo, 56/58 RS (+) re-tested were found still positive; 27 pts with LR/HR-RS still harboured the same genotype; 2 LR became negative; 5/9 RS-negative became positive, 3 with HR-HPV.

Among 71 PhS evaluated, 17 (24%) were positive (5 HR-HPV), 15 with concurrent RS (+), often with a different genotype.

81% of pts receiving antiretroviral therapy (66% of total) and 90% of naive pts (34% of total) were found RS(+). No significant differences were observed in HR-HPV prevalence among these groups.

92 pts were evaluated for oral HHV8 shedding. 12 pts were HHV8(-)/RS-HPV-neg, 55 HHV8(-)/RS-HPV(+) and 25 (27%) HHV8(+)/RS-HPV(+); 12 associated with HR/LR-HPV. 66% of HHV8-pos were HIV treated.

Conclusions: A high prevalence of HPV infection was found in RS from HIV(+) MSM, with HR/LR types in at least 76% of positive RS and in 61% in the entire cohort, irrespective of HAART treatment. Moreover, the persistence over 6 mo of the same HPV-types in RS was commonly observed, together with HPV infection in pharynx. HHV8 oral shedding was correlated with HPV infection. These findings suggest a possible multiple source of acquisition and spreading of HPV to sexual and non-sexual contacts. Careful survey for HPV and HHV8 infection should be carry out in HIV-MSM, a population at high risk of developing HPV- and HHV8-related cancers. These co-infections suggest repeated sexual unprotected contacts.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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