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Molecular detection of 12 viruses and subtypes by Luminex respiratory viral panel in respiratory specimens from children

Abstract number: O500

Selvarangan R., Selvaraju S., Baker D., Estes K., Hays L., Abel D., Hiraki S.

Objective: Luminex respiratory viral panel (RVP) is a multiplex PCR capable of detecting and differentiating twelve different respiratory viruses and their subtypes; influenza A (Flu A) (subtypes H1 and H3), influenza B (Flu B), Respiratory syncytial virus (RSV) (subtypes A and B), Adenovirus (AdV), Parainfluenza 1 (PIV 1), Parainfluenza 2 (PIV 2), Parainfluenza 3 (PIV 3), human metapneumovirus (hMPV) and Rhinovirus (RhV). The aim of this study was to evaluate the analytical performance characteristics of RVP assay and to evaluate its ability to detect respiratory viruses from nasopharyngeal aspirates obtained from children.

Method: Analytical sensitivity, specificity, accuracy and precision of the Luminex RVP assay were determined using control viral stocks and respiratory specimens previously tested by Rmix shell vial culture.

Result: Luminex RVP assay reliably detected ATCC viral stocks of Flu A, Flu B, RSVB, RhV and PIV3 in the range of 10e-2 to 10e-4 TCID50/ml. No cross reactivity was noted with CMV, HSV, HHV6, EBV, VZV, PIV4, cornoavirus 229E and OC43. Among 146 respiratory specimens previously characterised by culture 138 specimens were accurately detected with overall accuracy of 95%. The median coefficient of variation in mean fluorescent index values of signals from replicate analyses of influenza A, B and RSV was 9% (7% to 25%). The 146 clinical specimens tested by RVP assay included 109 culture positive and 37 culture negative specimens. Respiratory viruses isolated from the culture positive specimens include the following; 19 AdV, 11 Flu A, 10 Flu B, 19 RSV, 9 PIV1, 6 PIV2, 5 PIV3, 19 hMPV and 14 RhV. RVP assay detected all of the respiratory viruses except one each of RSV, PIV1 and PIV2 virus with overall sensitivity ranging from 88% to 100% for the different respiratory viral groups. Among the 37 culture negative specimens 20 respiratory viruses were detected by RVP of which 15 were subsequently confirmed by repeat analyses.

Conclusion: Luminex RVP assay is a highly sensitive and specific test useful in the detection of commonly encountered respiratory viruses in respiratory specimens. The addition of RVP assay to the viral testing algorithm of respiratory infections in children provides rapid results, improves diagnostic yield and may result in decreased antibiotic usage, reduced diagnostic testing and reduced hospital stay.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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