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Construction of a recombinant bacmid containing papillomavirus type16-L1 gene

Abstract number: R2546

Abdoli A., Soleimanjahi H., Fotouhi chahooki F., Teimoori A., Fazeli M., Farzaneh pour F., Shenagari M.

Objectives: Major capsid protein L1 of HPV-16 intrinsically of assembling and producing viral-like particle (VLP) in vitro.

Methods: HPV16-L1 gene extracted from paraffin embedded infected cervical tissues of patients who suffered from cervical cancer. This gene was inserted into pFast HTA plasmid. After the confirmation, the recombinant plasmid was transfected into DH10 E.Coli containing Bacmid and helper plasmid.

Results: It was observed recombinant Bacmid colonies turned to white colonies and non-recombinant colonies remained blue in absence of X-gal and IPTG. For confirming the recombinant Bacmid production, an in-house designed PCR was applied.

Conclusion: This research has lead to the production of recombinant Bacmid which can replicate in insect cells and produce L1 protein. This protein has the power of assemblage and construction of VLP.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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