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Diagnosis of Mycoplasma pneumoniae: results of a prospective study in Tunisia

Abstract number: R2539

Touati A., Achour W., Ben Hassen A.

Objectives:Mycoplasma pneumoniae is an important human respiratory tract pathogen; it causes 15 to 20% of community-acquired pneumonia in older children and adults and a variety of respiratory tract infection in younger children. The aim of our study was to diagnose and to determine the overall incidence of M. pneumoniae infection in young children.

Methods: a prospective study was conducted from November 2005 to October 2007. It concerned 273 neonates and infants hospitalised in the intensive care unit in children's hospital in Tunis and suffering from respiratory problems. We collected 285 different specimens, essentially from respiratory tract, placed into transport medium (2SP). Samples were tested by 16S rRNA gene- and P1 adhesin gene-based PCR and by inoculation in Hayflick modified broth medium supplemented with glucose. Two hundred and ninety-one sera obtained at the same time were used for detection of IgM and IgG antibodies using ELISA-PLATELIA M. pneumoniae TMB tests (BioRad®). Typing of M. pneumoniae clinical isolates was carried out by a PCR-RFLP method using HpaII restriction endonuclease.

Results: samples were distributed as follow: 174 nasopharyngeal aspirates, 42 throat swabs, 31 nose swabs, 25 tracheal aspirates, 7 bronchoalveolar lavages, 4 cerebrospinal fluids, 1 sputum, 1 articular fluid and 1 pleural fluid. We totally isolated 5 M. pneumoniae clinical isolates; all strains belonged to subtype I.

Fourteen patients (10 infants and 4 neonates) could be diagnosed with certain M. pneumoniae infection: 4 had positive culture, one had positive nasopharyngeal aspirate by PCR and culture, one had positive throat swab-PCR and serology (positive IgM and moderate IgG), one with positive IgM and high IgG titer, 4 had positive IgM associated with low or moderate or negative IgG and 3 with seroconversions.

Conclusion: our study showed a low incidence (5.12%) (14/273) of current M. pneumoniae infection. All M. pneumoniae clinical strains were of subtype I. Culture, PCR and serology tests are complementary for the confirmation of diagnosis.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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