Catheter-related bacteraemia with Methylobacterium species
Abstract number: R2517
Holt H.M., Skov M.N.
Objectives: Methylobacterium is an aerobic, slow-growing, Gram-negative bacillus rarely found in human, clinical specimens.
We present three cases of bacteraemia with Methylobacterium species and the results of phenotypic characterisation, antibiotic susceptibility testing and 16S rRNA gene sequencing.
Methods: Clinical records from three patients with Methylobacterium infections were obtained. The BacTec bloodculture system was used. Identification was made by standard methods, VITEK®2 GN card and direct sequencing of the 16S rRNA gene. Susceptibility testing was performed by direct agar diffusion with Rosco Neo-Sensitabs® and by E-test®.
Results: Three cases of bacteraemia with four isolates of Methylobacterium species in pure culture are presented. Two patients were immunocompromised and received chemotherapy for acute leukaemia; one patient had pancreatitis and multiorgan failure. All patients had a central venous catheter as the most likely focus of infection. All isolates of Methylobacterium were growing slowly with a time to detection of 35 days in the BacTec® system and two days incubation after subculturing. Three of the four isolates were resistant to all betalactam antibiotics inclusive meropenem but susceptible to tetracycline and aminoglycosides. Comparison of the 16S rRNA gene sequences showed different patterns and a common source of infection therefore was unlikely.
Conclusion: Catheter related infections with Methylobacterium are seen in Denmark. The infection might be under-diagnosed as Methylobacterium is slow-growing and not always visible in the initial Gram-stain. The genus is often resistant to meropenem and other betalactam-antibiotics but susceptible to aminoglycosides and tetracyclines
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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