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Genotyping of Mycobacterium tuberculosis MDR and XDR strains in Lima, Peru

Abstract number: R2501

Tello C., Borja N., Gavilán R.G., Ruiz J., del Valle L.J., Vargas M., Ramirez P., Garcia de la Guarda R.

Objective: To characterise the genotypes of Mycobacterium tuberculosis both multidrugresistant (MDR), expensive drug resistant (XDR) and susceptible to drugs strains.

Methods: Forty-nine patients with TB were included in the study. The genotypes of the M. tuberculosis isolates were established by PCR using the primer Mtb2 (5'-CGG-CGG-CAA-CGG-CGG-CA-3') in combination with primers sited at inverted repeats flanking IS6110.

The susceptibility to isoniazid, rifampicin, streptomycin, ethambutol, kanamycin, para-amin-salicylic acid, tioacetazon and pyrazinamide was studied.

Results: The 36.7% of the patients presents isolates susceptible to all the drugs tested. The 12.9%, 9.7%, 9.7%, 48.4% and 19.4% were resistant for one, two, three drugs, MDR and XDR, respectively. The resistance levels to isoniazid, streptomycin and rifampicin were the most relevant, ranged between between 47% and 49%.

The 16% of the patients were VIH+, three out of them presents MDR strains, while no XDR isolate was isolated from these patients.

When considered the patients with MDR strains the 80% reported previous TB.

Upon PCR-based genotyping analysis of 49 M. tuberculosis strains, the presence of 42 different fingerprints and 10 different clusters were established, the number of bands for each fingerprint ranged from 7 to 1 band(s). The MDR isolates were non-included in a specific cluster

Conclusions: An High number of MDR isolates were detected. Additionally, the recovered isolates presents a low level of clonality, showing a great variety of genotypes and a low level of clustering, this phenomena affects specially the MDR isolates.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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