Prevalence of parvovirus B19 infections among patients with sickle cell anaemia and thalassaemia in Saudi Arabia
Abstract number: R2495
Alzahrani A.J., Obeid O.E.
Objectives: The present study was aimed at serological assessment of parvovirus B19 infection among sickle cell anaemia and thalassaemia patients in Al-Khobar and Dammam, Saudi Arabia.
Methods: the methods used are clinical assessment, serological measurement of parvovirus B19 antibodies using ELISA, viral DNA using PCR and an interview-based questionnaire.
Results: the age of the patients included in the study (N=138) ranged from 6 months to 61 years (mean 28 years). 40 samples (28.4%) have detectable levels of Parvovirus B19 IgG. 6 samples (4.3%) have detectable levels of Parvovirus B19 IgM antibodies. All IgM-positive samples had detectable B19 parvovirus DNA by PCR. Samples of sickle cell disease patients (N=138) were analysed for antibodies against Parvovirus B19 using the novel western blot assay (recomblot assay). 46 samples (33.3%) have detectable levels of Parvovirus B19 antibodies by recomblot assay.
Conclusion: establishing the extend of parvovirus B19 infection in sickle cell anaemia and thalassaemia patients will help in proper management of aplastic crisis in such patients and will also be useful for epidemiological purposes.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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