Detection of human papillomavirus in HIV-positive Venezuelan patients
Abstract number: R2476
Avila M., Correnti M., Cavazza M.E., Reyes O., Alfonso R.
Objective: The aim of this work was to evaluate the association between the HIV infection and the genotypes of HPV in genital and anal lesions.
Methods: Patients: Thirty eight patients with a diagnosis of HIV infection (thirty seven men and one woman) were rolled after informed consent and complete physical examination.
Nineteen biopsies of the genital area and eighteen anal swaps were obtained for HPV test. The amplification of 450 Pb fragment of HPV DNA included in the open reading frame of L1 region was performed using the degenerate primers MY09 and MY11. To determine specimen adequacy, the GH20/ PC04 human beta-Globins target was co amplified with HPV sequences. A known positive specimen and a negative specimen were included in each assay as controls. The typing of HPV was determinated by MPCR Amplification (Maxim Biotech, Inc.) that simultaneously detects the HPV types 6, 11, 16, 18 and 33. The MPCR DNA products were separate eletrophoretically on a 2% agarose gel containing ethidium bromide. The CD4 count and viral load were evaluated in 18 patients of the study group.
Results: HPV infection was detected in 35 HIV infected patients (95%). The 19 biopsies of genital area were histological diagnosed as condylomata acuminate. The location of these lesions was observed with more frequency in the perianal area (57.89%), followed of penis (36.84%) and vulva (5.27%). The HPV was detected in all specimens of condylomata acuminate, the type 11 was observed with more frequency (63.16%), followed by the type 6 (21.05%), three cases (15.17%) was none typified and neither case was observed co-infection with types of high risk oncogenic. In contrast, HPV 16 genotype was found in 16/18 of the anal swaps samples.
We did not find any correlation between CD4 cell count, HIV RNA viral load and the presence of specific HPV genotype.
Conclusion: In this study the results show a clear association of the low oncogenic risk types 6 and 11 and the development of the genital warts. Our data showed that in our population of HIV infected men, there is a high prevalence of HPV 16 genotype in anal samples. A screening programme to detect anal squamous intraepithelial lesions in high-risk individuals may be of value to prevent anal cancer.
Grants: FONACIT G-2005000408.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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