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Isolation from blood cultures and susceptibility testing of Candida sp. strains during three years in a tertiary hospital in Greece

Abstract number: R2468

Orfanidou M., Ganteris G., Karanika M., Ziva K., Petropoulou D., Malamou-Lada H.

Objectives: To study the isolation from blood cultures and the susceptibility of Candida sp strains to antifungal drugs in a tertiary Hospital in Greece, during a three year period (1/1/2004–31/12/2006).

Methods: During the study period blood cultures from 15723 patients were sent to the laboratory. The vials of the blood cultures were incubated in BACTEC 9240 (BD) and BacT/ALERT 3D (bioMerieux) automated systems. Positive vials were subcultured on conventional media. The strains were identified by VITEK 2 (bioMerieux) automated system. Susceptibility testing was performed on RPMI agar (E&O) by E-test (AB Biodisk).

Results: During three years' period, blood cultures from 2605 patients were found to be positive (2605/15723, 16.57%). Candida sp strains were isolated in 71/2605 (2.7%) of these patients. The origin of the strains was from Internal Medicine Department 32/71 (45.1%), Surgical Department 24/71 (33.8) and Intensive Care Unit (ICU) 15/71 (21.1%). Candida albicans was the predominant strain (29/71, 40.8%), followed by C. parapsilosis (15/71, 21.1%), C. glabrata (13/71, 18.3%), C. tropicalis (7/71, 9.8%), two strains of C. famata, two strains of C. lusitaniae and C. krusei, C. dubliniensis and Candida sp one strain each. Susceptibility was tested in 50 strains. The resistance rate of the isolated strains was: itraconazole 29%, fluconazole 22%, voriconazole 7%, fluocytosine 6%, while there was no resistance to caspofungin and amphotericin B.

Conclusions:

1The rate of candidaemia in our hospital is approximately 3% of the patients with positive blood cultures.

2C. albicans represented 40% while non-albicansCandida composed 60% of the total.

3There was a high resistance rate to itraconazole and fluconazole, but also resistance of lower rate was observed to voriconazole and fluocytosine, while there was no resistance to caspofungin and amphotericin B.

4In cases of candidaemia antifungal therapy according to the susceptibility test of the isolated strains is warranted.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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