Usefulness of enzyme immunoassay as a screening method in syphilis serology

Abstract number: R2404

Dopico E., Ros J., Grenzner E., Vinuesa T.

Objective: The aim of this study is to evaluate the usefulness of the enzyme immunoassay (EIA) method bioelisa SYPHILIS 3.0 versus RPR and TPHA tests, in laboratory diagnosis of syphilis.

Methods: EIA was performed in 87 serums obtained in October and November 2007, selected by their positive result in RPR, TPHA or FTA-Abs in order to know if EIA test was able to screen them as positive samples. In our laboratory, FTA-Abs was performed only when RPR or TPHA test was positive. We considered true positives results when RPR and TPHA were positive, or RPR negative and TPHA and FTA-Abs positive and false positive when RPR or TPHA were positive and FTA negative.

EIA results were compared to a combination of RPR/TPHA/FTA-Abs that allowed considering true or false positive results.

Results: EIA versus current syphilis serology tests shows a sensitivity of 93.3% and a specificity of 97.6%.

Conclusion: Although EIA may be a useful screening method in laboratories that perform a large number of tests in which automation becomes absolutely necessary, positive tests need to be confirmed by classical methods of nontreponemal and treponemal tests. Nontreponemal test are still useful as a measure of syphilis activity and in monitoring drug response.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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