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Evaluation of the new VITEK-2 ANC identification card in a clinical laboratory

Abstract number: R2396

Mory F., Alauzet C., Riegel P., Matuszewski C., Chatellier S., Lozniewski A.

Objectives: The purpose of this study was to evaluate the ability of the new VITEK2 ANC card (bioMérieux, Marcy l'Etoile, France) to identify anaerobic and coryneform bacteria in comparison to reference methods.

Methods: a total of 319 strains belonging to the 63 species of anaerobic and coryneform bacteria listed in the ANC card database were investigated. Strains included 263 anaerobic clinical strains isolated in the laboratory of Bacteriology, University Hospital Centre, Nancy, France and 56 coryneform clinical strains isolated in the laboratory of Bacteriology, University Hospital Centre, Strasbourg, France. These isolates were previously identified by conventional methods. The new VITEK 2 ANC cards were used according to the manufacturer's recommendations. The cards were filled with organism suspensions prepared in 0.45% aqueous NaCl to turbidity equivalent to a McFarland #3 standard. Inoculated cards were incubated in the VITEK2 for approximately 6 h. Identification results were generated by a computer-assisted algorithm. In the case of conflicting results, 16S rRNA and rpoB gene sequencing methods were used for genetic identification.

Results: The new VITEK 2 ANC card was able to identify 314 (98.4%) strains to the genus level and 281 (88%) to the species level. Nine (2.8%) strains were misidentified and two (0.6%) could not be identified.

Conclusion: The new VITEK2 ANC appears to be a useful and reliable method for the rapid identification of anaerobic and coryneform bacteria.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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