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CagA and other key genes in the cag pathogenicity island of Helicobacter pylori isolated from dyspepsia patients

Abstract number: R2364

Jafari F., Shokrzadeh L., Baghai K., Dabiri H., Bolfion M., Sahebekhtiyari N., Zojaji H., Mashayekhi R., Zali MR.

Objective:Helicobacter pylori is a bacterium associated with upper gastrointestinal diseases in humans. Several studies have tried to establish an association between known virulence markers and clinical outcomes, in many cases the results has been conflicting. Cytotoxcin associated gene A (cagA), E (cagE) and certain vaculating cytotoxin (vacA) genotypes are associated with peptic ulcer disease (PUD). The present study compared the distribution of vacA allotypes and cag PAI status in Iranian patients isolates.

Methods: From November 2006 to July 2007, two hundred and ten patients referred to the Taleghani hospital for endoscopy.197 gastric biopsy specimens of 169 none ulcer disease (NUD) and 28 peptic ulcer disease (PUD) were distinguished qualify for next steps. The samples were cultured by standard methods. PCR was performed for ure C (glmM) gene to identify Helicobacter pylori strains. Isolation of cagE, cagM, cagG, oipA and vacA allotypes were done by PCR.

Results: Among NUD and PUD, H. pylori was isolated in 148 of 169 (75.1%) and 21of 28 (75%) respectively. PCR amplification detected cag A gene in 103 (69.6%) of NUD patients and in 66.7% of PUD patients. cagT, cagG, cagM, cagE and oipA genes were observed in H. pylori isolates from NUD patients in 29.7%, 10%, 0.2%, 39.4% and 50% respectively. Also these genes were found in 23%, 9%, 33% and 42% of PUD patients respectively. Among analysis of vacA allotypes, s1 and m2 genotype were predominant types among H. pylori strains (36.2%). CagM was observed in 4% of the H. pylori isolates in PUD and was more than isolated strains in NUD(0.2%).

Conclusion: The data show that, infection by H. pylori is an important cause of gastritis in Iranian patients. Also the results note that, there was no association between cagA, cagG, cagM, cagE and oipA genes and clinical outcomes. The result of this study showed none of the markers are helpful in predicting the clinical presentation of a H. pylori infection, however, for full confirmation of our data and explanation of possible factors of H. pylori to the pathogenesis of the diseases further data to be collected and evaluated.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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