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Four-year survey of meticillin-resistant Staphylococcus aureus producing Panton-Valentine leukocidin in northern Greece

Abstract number: R2343

Panopoulou M., Alepopoulou E., Grapsa A., Batarli A., Petinaki E., Ktenidoy Kartali S.

Objectives: Meticillin-resistant Staphylococcus aureus is a major cause of hospital and community-acquired infections, such as surgical wound, skin and soft tissue infections and pneumonia.

The aim of this study was to define the prevalence and clonal distribution of PVL-positive MRSA among patients with staphylococcal infections in northern Greece.

Methods: Five hundred twenty eight Staphylococcus aureus isolates were collected during the 4-year period between January 2004 and September 2007 at the University Hospital of Alexandroupolis, Greece. Isolates were identified by conventional methods and the automated system VITEK 2 (BioMerieux, France). Susceptibility tests were performed by the disk diffusion method and determination of MIC by VITEK 2. The presence of PVL gene was detected by PCR, while the clonal relatedness was tested by PFGE.

Results: One hundred seventy three isolates(33%) were meticillin resistant. Among them, seventy three (42%), were resistant to kanamycin, tetracycline, intermediate susceptible to fusidic acid and carried the PVL gene. PFGE analysis revealed that all these isolates belonged to the clone C (ST 80), that predominates in Europe. The distribution of PVL positive strains were 43%, 51%, 35%, 37% for the years 2004, 2005, 2006, 2007 respectively.

Conclusion: In Greece the majority of PVL-positive strains express a common phenotype of antimicrobial agents(resistance to oxacillin, kanamycin, tetracycline and fusidic acid). Thus, isolates recovered from musculoskeletal infections with this phenotype must be examined for the presence of PVL gene, in order to establish prevention measures.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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