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Panton-Valentine leukocidin positive, t008 spa-type meticillin-resistant Staphylococcus aureus in a Romanian western county hospital

Abstract number: R2311

Codita I., Oprea M., Szmal C., Dragulescu E.C., Trasca M., Brehar Cioflec D., Chicin G., Serban R., Pistol A.

Objectives: To preliminary evaluate the possible hospital isolation of the USA300 genotype community-associated meticillin resistant Staphylococcus aureus strain in a Romanian Western county hospital.

Methods:Staphylococcus aureus strains were identified using conventional, API and automate methods (VITEK 2). Meticillin-resistance was screened by the cefoxitin disc method and confirmed by mecA PCR. MecA gene was detected by a triplex PCR variant optimised in our laboratory, detecting concomitantly the nuc gene coding for the thermonuclease and the lukS/F genes coding for the Panton Valentine leucocidin (PVL). PFGE was performed according to the HARMONY protocol. Spa-typing was done by DNA sequencing analysis of the protein A (spa) gene hypervariable region according to the SeqEUNet.org recommended protocols.

Results: 91 S. aureus strains have been analysed at the National Nosocomial Infections and Antimicrobial Resistance Laboratory, National Institute of Research-Development for Microbiology and Immunology "Cantacuzino", Bucharest, including the SeqEUNet.org 2006/2007 6 months study and all ICU strains received during the January 2006–June 2007 interval. Among these, 4 strains received from a Romanian Western county hospital isolated in January/February 2007 harboured the t008 spa-type, similar with the spa-type of the USA300 community associated genotype, which has been recently discovered in Germany (Witte et al. – JAC Advance Access published online on October 13, 2007). Two of the 4 strains were MRSA. These MRSA strains proved lukS/F positive. The two strains were isolated from 2 patients, 3 months and 14 years old, respectively, receiving healthcare in the ICU of the Western county Romanian hospital. Both t008, PVL positive MRSA strains harboured the same pulsotype. The Romanian strains pulsotype looks very similar with the USA300 pulsotype (Johnson et al – Em. Inf. Dis., 13, 8, 2007).

Conclusion: The t008 spa-type MRSA strains received from a Romanian Western county hospital could be related with the USA300 genotype strain based on the presence of lukS/F genes, spa type and PFGE profile. Further molecular studies, including SCCmec typing and searching for the arginine catabolic mobile element (ACME) determinant will help us to clearly establish the clonal relatedness of these 2 identical Romanian strains with the USA300 genotype community associated strain.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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