Staphylococcal cassette chromosome mec profiles of Staphylococcus aureus isolated in 13 Korean hospitals

Abstract number: R2309

Kim J.S., Song W.K., Kim H.S., Eom J.S., Lee K.M.

Background: There has been a nationwide increase in infections caused by Staphylococcus aureus resistant to multiple antimicrobial agents in Korea. The prevalence of MRSA in S. aureus in tertiary-care hospital is nearly 70% since the mid-1990s. We assessed the staphylococcal cassette chromosome mec (SCCmec) profiles and Panton-Valentine leukocidin (PVL) on Korean nationwide collection of S. aureus strains from tertiary-care hospitals in Korea.

Methods: We collected a total of 250 clinical isolates of S. aureus from 13 clinical laboratories over the country during 2 months in 2007. Meticillin-resistant S. aureus (MRSA) isolates were confirmed by oxacillin susceptibility test and PCR detection of mecA gene. SCCmec typing was performed by multiplex PCR. Panton-Valentine leukocidin (PVL) gene was also detected by PCR.

Results: Among 250 isolates, 175 strains (70.0%) were MRSA. Of the 175 MRSA isolates, 0 (0%), 129 (73.7%), 31 (17.7%), and 15 (8.6%) isolates belonged to SCCmec types I, II, III, and IV, respectively. The most prevalent subtype was SCCmec type IIb (n = 109, 62.3%). SCCmec type IVa strains known as community-associated MRSA in Korea were 10 (5.7%) isolates. None of the MRSA isolates were PVL-positive.

Conclusions: SCCmec type II strains are the most frequent SCCmec type in Korea, followed by SCCmec types III and IV. SCCmec subtype IIb strains were detected in 12 hospitals throughout the country and have increased in Korea, compared to previous study (62.3% in 2007 vs. 33.7% in 2002). SCCmec type I strains or PVL-positive S. aureus strains were not detected, though they are often isolated in Europe.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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