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The prevention of microbial biofilm formation on net prosthetic appliance for hernioplasty

Abstract number: R2266

Afinogenova A., Afinogenov G., Tikhilov R., Mironenko A., Postrelov N., Basin B., Koltsov A.

Objectives: To evaluate the ability of net prosthetic appliance to prevent the microbial biofilm formation.

Methods: Net prosthetic appliance is made of lavsan polyfilaments. Antimicrobial effect was attained in vitro when the composition of mixed high dispersed silver with high-molecular weight polyvinylpyrrolidine (PVP) was placed on polymeric net. Cell Wells™ 6-Well with Lid were used. Control – net without mixed composition. The piece of net was placed on the plate bottom with nutrient medium, then infect with S.aureus ATCC 6538 was added. The plates were incubated at 37°C, 1 week, 2 weeks or 3 weeks. The CFU/ml as a testing parameter was evaluated during the experiment. In vivo the net prosthetic appliances were placed subcutaneous on guinea-pigs in conditions of microbial contamination. Experimental animals were disabled on the 1st, 3d and 5th day of experiment. Then the pieces of net prosthetic appliances were placed on Petri dishes and incubated at 37°C, 24 h; after what the CFU/cm2 of each example were evaluated. The tissue histological examination was performed in surgical zone in 10–15 days.

Results: In vitro control plates showed solid infect growth after 24 h and the increasing level of CFU during 3 weeks up to 109 CFU/ml. Experimental examples showed no CFU in 1 and 2 weeks, during the 3d week 102 CFU/ml were obtained. In vivo net prosthetic appliance with tested composition helped to reduce the content of S.aureus in wound. Control nets possessed 103 CFU/cm2 on the 1st day; 106 CFU/cm2 on the 3d day and 109 CFU/cm2 on the 5th day. The examples of silver-net tested after 1st, 3d and 5th day were sterile and possessed antimicrobial effect against S.aureus by agar diffusion method. Their prolonged antimicrobial effect was obtained after more than 5 days as well, this is necessary for optimisation of wound reparative processing. No pus process was in each experimental wound where silver-nets were placed. Control animals possessed the signs of sub-clinical festering.

Conclusions: High-molecular weight polyvinylpyrrolidine mixed with silver penetrates to lavsan polyfilaments pores leading to pseudo-monofiber formation. Tested composition PVP and silver is biodegradable in 1 week after net application. The net pores cleared that way are ready for conjunctive tissue growth. Net prosthetic appliance with antimicrobial composition was successfully applied in different clinics in Russia.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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