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Inhibitory effect of hyperimmune LPS antiserum in attachment of E. coli O157:H7 to HEP-2 cell and murine model

Abstract number: R2252

Moradi Bidhendi S., Khaki P., Pourbakhsh S., Sattari M., Heidari M.

Objective: Enteroheamorragic E. coli(EHEC) strain of serotype O157:H7 is associated with humans disease. The organism have also been associated with a spectrum of sporadic and endemic human illnesses ranging from nonbloody diarrhoea to the haemolytic uremic syndrome. This work was carried out to study of inhibitory effect of hyperimmune LPS antiserum in attachment of bacteria to HEP-2 cell and murine model.

Methods: Bacterial strain was isolated from human. Hyperimmune LPS antiserum was prepared by injecting to rabbit. Different dilution of antiserum prepared. These dilution added to the suspension of bacteria(2×108 cfu/ml) for 30 minutes then added to the HEP-2 cell and after 3–6 hours the cells examined with staining and light microscope. For murine model these mixture gave to mice with gavage and the inhibitory attachment of these dilution attained with staining and electron microscope of intestinal tract of mice.

Results: The result showed that 1/1280 dilution can prevent the attachment of the bacterium to HEP-2 cell. For murine model the result showed 1/640 dilution of antiserum was inhibitory.

Discussion: Our results suggest that the prepared antiserum can use to prevention of bacterial attachment and may be useful for control of the disease that cause with this bacteria.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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