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Relationship between Helicobacter pylori babA and babB status with other virulence factors and their correlation with disease outcome in Iran

Abstract number: R2248

Saberi Kashani S., Douraghi M., Talebkhan Y., Bababeik M., Esmaeili M., Mohagheghi M.A., Eshagh Hosseini M., Mohammadi M.

Objectives:Helicobacter pylori (Hp) is an important human pathogen associated with gastrointestinal diseases such as gastritis, peptic ulcer disease (PUD) and gastric cancer. A number of pathogenic factors have been described for this bacterium, and some of them have been proposed as predictive markers of the clinical outcomes. However, with the exception of the cag and vacA status, there is no global consensus regarding the role of the other introduced virulence factors. Therefore, the aim of this study was to investigate the status of Hp strains regarding the Hp babA and babB (blood group antigen binding) adhesins and assess any existing association between the status of these genes and clinical outcomes in Iranian patients.

Methods: Hp virulence genes were amplified by means of gene-specific polymerase chain reaction in clinical isolates of Hp from 72 Iranian patients (16 GC, 12 PUD, 44 NUD patients).

Results: Eleven categories of genotypes were identified with the following frequencies: Group 1: A+B+/cagA+/s1 (38.0%); Group 2: A+B+/cag+/s2 (2.8%); Group 3: A+B+/cag-/s1 (1.4%); Group 4: A+B-/cag+/s1 (7%); Group 5: A+B-/cag+/s2 (2.8%); Group 6: A-B+/cag+/s1 (22.5%); Group 7: A-B+/cag+/s2 (14.1%); Group 8: A-B+/cag-/s1 (1.4%); Group 9: A-B+/cag-/s2(4.2%); Group 10: A-B-/cag+/s 1(2.8%); Group 11: A-B-/cag+/s2 (2.8%). Frequency of Group 1 in GC, NUD and PUD patients is 75%, 27.3% and 27.3% respectively and Group 7 is prevalent in 36.4% of PUD patients. babA prevalence in GC, NUD and PUD patients was 75%, 47.7% and 33.3% and babB prevalence was 93.8%, 77.3% and 91.7% respectively. 75% of Hp isolates were babA/babB double positive in GC patients.

Conclusion: Frequency of babB is higher among Iranian GC and PUD cases but babA frequency is limited to GC cases. Infecting strains from Groups 1 and 7 were found to be associated with GC and PUD respectively. Co-presence of cagA, s1vacA, babA and babB may work synergistically in exacerbating the resulting inflammation which may create grounds for development of intestinal metaplasia and eventually GC, whereas co-existence of cagA, s2vacA, babB may cause susceptibility to PUD development. In GC cases presence of babA/babB double positive are significantly prevalent (p = 0.044). Application of this analysis on additional samples will allow for a more concrete conclusion.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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