Comparison of Austrian, Hungarian and Macedonian meticillin-resistant and meticillin-sensitive Staphylococcus aureus strains in relation to accessory gene regulator type
Abstract number: R2245
Kocsis E., Lagler H., Pesti N., Stich K., Kristóf K., Nagy K., Cekovska Z., Graninger W., Rozgonyi F.
Objectives: The purpose of this study was to examine the presence of genes responsible for different accessory gene regulator (agr) types in Staphylococcus aureus strains isolated from invasive clinical samples, and compare according to country origin and meticillin resistance.
Methods: Classical microbiological methods were used for the phenotypical identification of the strains. By detecting the genes encoding thermostabile endonuclease (nucA) and 23S rRNA the genetic confirmation of the species of the strains was done. According to the presence of the mecA gene 48 Austrian (AT) meticillin-resistant S. aureus (MRSA) and 128 meticillin-sensitive S. aureus (MSSA), 110 Hungarian (HU)MRSA and 94 MSSA, 73 Macedonian (MK) MRSA and 29 MSSA strains were studied. The genes responsible for four different agr types were detected by multiplex polymerase chain reaction. The pulsed-field gel electrophoresis of the strains was also done to confirm the heterogenic feature of the bacterial groups.
Results: The agr1 gene was detected in 46%, 47% and 93% in AT, HU and MK MSSA strains, while in 40%, 20% and 97% in AT, HU and MK MRSA ones, respectively. Both the AT and HU MSSA strains harboured the agr2 gene in 34%, while the MK MSSA isolates carried this gene only in 7%. The agr2 gene carriage of the AT, HU and MK MRSA strains was 58%, 74% and 3%, respectively. The agr3 gene could be detected in AT, HU MSSA and MRSA strains in 15%, 13% and in 2%, 6%, respectively. The agr4 gene was harboured by only AT and HU MSSA strains in 5% and 6%.
Conclusion: The presence of the agr1 gene was significantly characteristic for the MK MSSA and MRSA strains compared to the AT and HU ones (p < 0.001). The frequency of the agr2 gene characterised significantly the HU MRSA strains (p < 0.01). Comparing all the MSSA strains with MRSA ones the prevalence of agr2 gene was significant for the MRSA isolates (p < 0.001). Our results may indicate alterations in the regulation of virulence factor genes.
Supported by ÖAD and TéT grant No.: A-19/02 and the Hungarian National Scientific Research Fund (OTKA) T046186.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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