Prevalence of Rotavirus, Adenovirus, Norovirus and Astrovirus infections by rapid EIA and ELISA assays in hospitalised French children

Abstract number: P2222

Tran A., Minette D., Leveque N., Jacques J., Lejeune B., Payan C., Andreoletti L.

Introduction: Rotavirus, Norovirus, Astrovirus and Adenovirus 40/41 have been recognised as the most important aetiological agents of childhood viral gastroenteritidis in industrialised countries. Little is known about the number and the distribution of these viruses in hospitalised children.

Objectives: To determine the prevalence and the distribution of viruses responsible for gastroenteritidis in hospitalised children.

Patients and Methods: From January to October 2007, a total of 623 faecal specimens were routinely collected from children with or without gastroenteritidis signs and who were born or admitted in two French hospital settings (Reims medical university centre; n = 280; mean age, 2.18±SD 3.64 years, range: 2 days – 12 years; Brest medical university centre n = 343; mean age, 1.62±2.4years, range: 4 days – 14 years). Rapid EIA analyses for rotavirus and adenovirus were performed at the time of hospitalisation (r-Biopharm), and new commercially available ELISA tests were used retrospectively for the detection of Norovirus and Astrovirus in frozen stool samples (Ridascreen, r-Biopharm).

Results: The overall rates of prevalence for Rotavirus, norovirus, adenovirus, and astrovirus were respectively 29, 12, 4.5 and 2.5% and they did not significantly differ between hospitals (P = 0.09). Dual virus infections were detected in 30 (4.8%) of the 623 study children and were associated with Norovirus in 19 (63%) infants including 4 prematurity cases. During winter, Norovirus infections accounted for 33% of all hospitalised gastroenteritidis cases at a time where rotavirus was epidemic, resulting in mixed Norovirus and rotavirus gastrointestinal tract infections. Of the 299 documented viral gastroenteritidis, 9 (3%) were identified as nosocomial infections that 4 occurred in prematurity cases.

Conclusion: These findings provide evidence that Noroviruses can be a leading cause of gastroenteritidis, and highlight the need to implement norovirus and astrovirus ELISA detection assays in association with rapid EIA rotavirus and adenovirus EIA detection for the clinical diagnosis and the nosocomial prevention of gastroenteritidis viral infections in paediatric departments.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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