Human Metapneumovirus and human Bocavirus in hospitalised children with acute respiratory infections

Abstract number: P2211

Zappa A., Canuti M., Amendola A., Pariani E., Colzani D., Ruzza M.L., Farina C., Podestà A., Perin S., Tanzi E.

Objectives: To determine the contribution in hospitalisation of both Human Metapneumovirus (hMPV) and Human Bocavirus (hBoV) infections among children younger than 3 years of age a retrospective study was performed. We collected and analysed respiratory samples from a paediatric population hospitalised at the San Carlo Borromeo hospital in Milan (Italy) between 2004 and 2007.

Methods: The study involved 186 (104 M, 82 F, mean age: 8.1 months) hospitalised children with acute lower respiratory symptoms (bronchiolitis, pneumonia and asthma). Informed consent was obtained from the parents of all the children who provided respiratory samples.

Pharyngeal swabs (PS) were collected from each patient and a form, containing information on demographic data, clinical diagnosis and epidemiological information, was filled.

HMPV-RNA and hBoV-DNA were extracted from PS and viral genome targets were detected by the amplification of 151bp of hMPV M gene and 354bp of hBoV NP gene.

Results: Overall, hMPV-RNA was detected in 19/186 (10.2%) PS samples and hBoV-DNA in 17/186 (9.1%) PS samples.

hMPV and hBoV infections were detected along with other respiratory viruses (Rhinovirus, Coronavirus 229E, Respiratory Syncytial Virus, Influenza A): the rate of co-infection was 26.3% for hMPV and 47.1% for hBoV. Ruling out the patients with co-infections, symptoms and clinical data were similar among hMPV and hBoV-infected children, even though 50% of hMPV-positive patients showed bronchiolitis.

Neither hBoV infections nor hMPV infections were identified in children over 2 years of age. Moreover, hMPV infection was more frequently observed in children with age leqslant R: less-than-or-eq, slant6 months respect to older children (p < 0.05). No differences of age profiles were observed in patients with hBoV infection.

The overall rate of hMPV and hBoV detection in respiratory samples varied slightly from year to year, although without any significant difference.

When the respiratory samples were stratified by month of collection, a statistical variation of the hBoV impact was observed. In fact, the highest frequency of hBoV (47.1% of total hBoV-positive samples) was detected in January. No seasonal variation in hMPV frequency was noted.

Conclusions: HMPV and hBoV has been associated with acute respiratory infection in young children. Our data suggest that both viruses are involved in episodes of hospitalisation in the paediatric population younger than 3 years of age with a similar frequency.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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