Passive immunisation protects cynomolgus macaques against Puumala hantavirus challenge
Abstract number: P2191
Klingström J., Stoltz M., Hardestam J., Ahlm C., Lundkvist Å.
Objectives: Hantaviruses cause two severe and often fatal human diseases: haemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Presently, there is no effective prevention available for HFRS or HPS. Here, we studied the effect of passive immunisation on the course of infection in cynomolgus macaques challenged with wild-type Puumala hantavirus (PUUV-wt).
Methods: A pool of serum, drawn from previously PUUV-wt-infected monkeys, was used for immunisation, and a pool of serum from the same monkeys that was obtained before infection was used as a control. Immunisations were administered three days before and 15 days after challenge with PUUV-wt. After challenge, monkeys were sampled once a week and analysed for markers of PUUV-infection.
Results: All three monkeys treated with non-immune serum became positive for PUUV-RNA in plasma and showed PUUV nucleocapsid-specific IgM-responses after challenge. In contrast, no PUUV-RNA or anti-PUUV specific IgM-response was detected in the three passively immunised monkeys. As seen in PUUV-infected humans, the control monkeys showed a marked decrease in the amount of platelets and increased levels of creatinine, IL-6, IL-10, and TNF after inoculation. In contrast, no marked changes in the amount of platelets were observed in the immunised monkeys and they did not show elevated levels of creatinine, IL-6, IL-10, or TNF after virus challenge.
Conclusion: The results show that passive immunisation in monkeys, using serum from previously hantavirus-infected individuals, can induce sterile protection and protect against pathogenesis. Convalescent-phase antibodies may represent a potential therapy that can induce immediate protection against HFRS and HPS.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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