Resistance to antifungal drugs of yeasts isolated from genital specimens

Abstract number: P2180

Botnarciuc M., Braga V., Voineagu L., Stoicescu R., Badea V., Barbu A.

Objectives: Identification of yeasts in genital specimens and testing their sensitivity to various antifungal drugs, in order to appreciate the rate of resistance to the most used of them. The study was realised during one year, September 2006-September 2007. All the specimens were collected from women with clinical symptomatology of infection present

Methods: We used CANDIFAST kit (International Microbio-France), which allows the identification of the main human pathogenic yeasts as well as testing their sensitivity to antifungal agents: polyene Nystatine and azoles Flucytosine, Econazole, Fluconazole, Miconazole. Due to the fact that the strains were isolated from vaginal samples, we tested them also to Ketoconazole, by disk diffusion method

Results: We analysed 160 specimens of vaginal secretions that had positive cultures on Sabouraud gelose. From 160 isolated strains, 142 were identified as Candida albicans, 12 strains identified as Saccharomyces sp., 4 strains as Candida glabrata and 2 strains as Candida krusei. Candida glabrata strains were resistant to Flucytosine, Miconazole, Fluconazole and Clotrimazole. Candida krusei were resistant to Nystatine, Flucytosine, Miconazole Fluconazole and Clotrimazole. Saccharomyces sp. did not presented resistance to the tested drugs. For Candida albicans strains the most important resistant rates were obtained for Ketoconazole (in 122 strains – 85.9%) and the lowest rate of resistance were for Nystatine and Econazole (in 12 strains – 8.6%).

Conclusions:Candida albicans remains the most frequent fungus isolated from genital infections. The sensitivity to antifungal drugs was good, in vitro, for Nystatine, Econazole, Clotrimazole Miconazole, Fluconazole and Flucytosine. We found important resistance in vitro to Ketoconazole. The difference between these two groups was significant.