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Susceptibility patterns of Helicobacter pylori paediatric strains in Spain

Abstract number: P2074

Agudo S., Alarcón T., Domingo D., Moreno M.J., López-Brea M.

Objective:Helicobacter pylori is a Gram-negative microaerophilic bacillus found in the human stomach and is associated with a wide range of digestive diseases. H. pylori resistance is an important factor in the efficacy of the treatment. The aim of this study was to determine the prevalence of antimicrobial resistance in H. pylori strains from paediatric patients. Isolates were tested against metronidazole, clarithromycin, ciprofloxacin, amoxicillin and tetracycline.

Methods: 193 samples were collected from gastric biopsies obtained by endoscopy to symptomatic children from January 2006 to November 2007. 83 out of 193 were positive for H. pylori. Biopsies were cultured according to standard microbiological procedures. The susceptibility was determined by E-test using 5% sheep blood agar (3–5 days incubation at 37° C in 10% CO2). Minimum inhibitory concentration was determined as the lowest concentration of drug able to inhibit visible growth, based on the CLSI and other previously published data.

Results: The resistance rate was 40.2% for metronidazole, 48.4% for clarithromycin and 11.4% for ciprofloxacin. All isolates of H. pylori were susceptibility to amoxicillin and tetracycline.

Table 1. Resistance rate

Conclusions: We found a high rate of clarithromycin and metronidazole resistance in our study. Due to the fact that clarithromycin resistance is specially critical and induce treatment failure, the use of this agent in empirical therapy should not be adviced in the treatment of Helicobacter pylori in our paediatric population. The data limit the therapeutic arsenal in Helicobacter pylori infection in children.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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