Study of Neisseria gonorrhoeae during a seven year period in a Spanish teaching hospital

Abstract number: P2071

Agudo S., Domingo D., Alarcon T., Navarro J., López-Brea M.

Introduction: The aim of this study was to analyse the antimicrobial susceptibility of Neisseria gonorrhoeae isolated from men with urethritis and to study the incidence of the microorganism during a seven year period in a Spanish teaching hospital.

Methods: One hundred and ninety three strains of Neisseria gonorrhoeae were isolated from 683 samples of urethral exudates, received at the Microbiology Department (Hospital Universitario de la Princesa, Madrid) from January 2001 to November 2007.

Urethral exudates were collected by standard procedures. They were examined by Gram stain and inoculated on Modified Thayer-Martin medium and Chocolate agar, and incubated at 37° in 10% CO2 until 48 hours. Penicillin, ciprofloxacin, tetracycline, ceftriaxone and spectinomycin suceptibility tests were performed using disc diffusion method.

Results: The percentage of positive cultures according to the number of samples received were 19.41%, 20.68%, 23.45%, 25.64%, 32.25% and 39.83% in 2001, 2002, 2003, 2004, 2005 and 2006 years respectivily. The susceptibility rate was 74.28% for penicillin, 99.42% for ceftriaxone, 72.14% for tetracycline, 63.12% for ciprofloxacin and 98.48% for spectinomycin. The susceptibility to ciprofloxacin decreased from 93.33% in the period of 2001–2003 to 67.93% in 2005–2007 years. Only one and two strains were resistant to ceftriaxone and spectinomycin, respectivily.

Conclusions: According to our work, resistance to penicillin, tetracycline and ciprofloxacin is high and can not be recommended for the treatment of gonorrhoea in our area. Ceftriaxone and spectinomycin should be considered the antimicrobial of choice. Quinolone resistance increased considerabily during the period of this study. Regarding to the rate of positive cultures, the number of gonorrhoea cases have increased from 19.41% cases in 2001 to 39.83 cases in 2006, probably due to changes in surveillance practices.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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