Multiple antibiotic resistance in Campylobacter spp. isolated from hospitalised children in Poland during 20022007
Abstract number: P2061
Rozynek E., Dzierzanowska-Fangrat K., Szczepanska B., Dzierzanowska D.
Introduction:C. jejuni and C. coli have been recognised as a major cause of foodborne gastrointestinal infections in humans. Many studies have shown a significant rise in resistance to fluoroquinolones, tetracycline and erythromycin in Campylobacter human isolates.
Objectives: The aim of this study was to analyse the prevalence and genetic mechanisms of antimicrobial resistance in Polish Campylobacter strains isolated from diarrhoeal children.
Material and Methods: A total of 163 C. jejuni and 31 C. coli isolated from stool samples of paediatric patients were analysed in the study. All samples were collected from children hospitalised in 6 large paediatric hospitals in Poland from 2002 through 2007. Isolation and identification were performed according to WHO recommendations and confirmed by the PCR assay. MICs of ciprofloxacin, tetracycline, ampicillin, gentamicin, and erythromycin were determined by the E-test. The presence of the tet(O) gene was detected by PCR. Thr-86-Ile mutations in the gyrA gene were identified by MAMA PCR, and confirmed by PCR-RFLP. The PCR-RFLP method was also used for the detection of A2074C and A2075G mutations in the 23S rRNA gene responsible for macrolide-resistance.
Results: A total of 65%C. coli and 63%C. jejuni isolates were resistant to at least one antimicrobial agent tested. The highest resistance rate was observed for ciprofloxacin (57%), followed by tetracycline (20%), ampicillin (16%), and gentamicin 2%. Only one C. jejuni strain was resistant to erythromycin. The results of phenotypic and genetic analyses of resistance to tetracycline were fully concordant. All tetracycline-resistant isolates possessed the tet(O) gene. All Campylobacter isolates resistant to quinolones by the Etest had Thr-86-Ile mutations in the gyrA gene detected molecular assays. However, these mutations were also found by both molecular methods in two isolates phenotypically susceptible to ciprofloxacin and nalidixic acid. Both isolates were further confirmed by direct sequencing to contain Thr-86-Ile mutations. The highest frequency of double resistance was found for ciprofloxacin and tetracycline (22%). Triple resistance was detected in 4% of isolates tested. Multiple antibiotic resistance rose significantly between 2005 and 2007.
Conclusions: Increasing multiple-resistance among human Campylobacter isolates observed in Poland is an important public health concern. This imply a need for constant monitoring of resistance.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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