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15-year survey of triclosan efficacy on supragingival plaque

Abstract number: P2058

Haraszthy V., Zambon J., Sreenivasan P., Haraszthy G., DeVizio W.

Triclosan (TCN) is a broad spectrum antimicrobial formulated in dentifrices and other oral hygiene preparations for daily use. In clinical studies, TCN dentifrices demonstrate significant improvements in oral health by reducing dental plaque and gingivitis.

Objectives: This report examined the antimicrobial efficacy of TCN on human supragingival dental plaque over a 15-year period.

Methods: Adults enrolled in these studies included those who had not undergone dental procedures or antimicrobial, systemic therapies for one month prior to study participation. The study was conducted in 11 separate periods with up to 10 subjects recruited for each period. Results from 92 adult subjects were analysed for this report. Supragingival plaque was collected from the entire dentition of each volunteer and placed in pre-reduced Ringer's solution. Each dental plaque sample was sonicated and then vortexed at maximal setting for one minute to disperse bacterial clumps prior to plating dilutions on solid media containing 7.5 or 25 mg/ml TCN. Controls included dilutions of each plaque sample plated on solid media without TCN. Colonies were enumerated from all solid media following incubation.

Results: Large numbers of viable supragingival bacteria were observed from all subjects on media without TCN. By contrast, dental plaque bacteria were substantially inhibited by TCN (p < 0.001). Bacterial inhibition with 7.5 and 25 mg/ml TCN ranged from 96–100% and 99.6–100%, respectively. For each concentration of TCN, a one-way ANOVA examined the percent susceptible bacteria as a response between each study period that served as a variable. No differences in microbial susceptibilities were observed between each period assessed over the 15-year duration (p > 0.3). For each TCN concentration, regression analyses indicate no alterations or trends in efficacy (p = 0.546 for 7.5 mg/ml TCN and p = 0.378 for 25 mg/ml TCN) over time.

Conclusions: Results demonstrate the significant efficacy of TCN on supragingival plaque bacteria over the 15-year duration with no alterations in microbial susceptibility. Since study participants were not screened based on their oral hygiene habits or use of oral hygiene formulations, the results demonstrate that TCN was effective on samples from a variety of subjects. Together, the results demonstrate that TCN represents a safe and effective agent for dental plaque bacteria.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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