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Factors involved in combined treatment with probiotic and fluorokinolone in Salmonella typhimurium murine infection

Abstract number: P1939

Truusalu K., Mikelsaar R., Kullisaar T., Aunapuu M., Arend A., Zilmer M., Mikelsaar M.

The aim was to detect the putative factors influencing the eradication of Salmonella Typhimurium (ST) by combined treatment with probiotic L. fermentum ME-3 (ME-3) and ofloxacin (OFX) in a murine model.

Material and Methods: Altogether 54 NIH line mice were investigated. Mice from Gr1, 2 and 3 (n = 12 in each group) were infected with a single dose of ST (105 cfu/ml) intragastrically; mice of Gr2 were treated with OFX (20 mg/kg) intragastrically daily. Mice of Gr3 were administered combined treatment with OFX and ME-3 (108cfu/ml) in the drinking water. The uninfected mice of Gr4 were administered ME-3, while Gr5 served as a control with PBS. The mice were sacrificed on Day 5 and Day 10. Blood, liver, spleen and ileum were seeded onto XLD and MRS media to detect ST. Histological slides were prepared from liver, spleen and ileum. GSSG peroxidase and reductase, the glutathione redox ratio (GSH/GSSG), lipid peroxides (LPO) and cytokines (IL-10, INFã and TNFá) in mucosa of ileum and liver were estimated.

Results:Salmonella Typhimurium was found in 4 infected mice from Gr1 and Gr2 (2 from blood, 4 from spleen and 1 from liver). The combined treatment eradicated ST from all investigated sites. Liver granulomas were found in 8 mice in both Gr1 and 2, while the addition of ME-3 reduced the number of mice with liver granulomas (p = 0.019).

The combined treatment caused significant reduction of LPO in mice of Gr3 as compared to Gr1 in the liver and ileum (p < 0.001; p = 0.005, resp.). ST infection equally increased the levels of all investigated cytokines as compared to control group. However, in the combined treatment group only the values of IL-10 significantly (p = 0.002; 0.001 resp.) exceeded these of TNFá and INFã.

Conclusion: In the experimental ST infection the combined treatment with probiotic L.fermentum ME-3 and OFX improves the eradication of ST from gut and reduces the number of granulomas in the liver of mice. The putative factors comprise the reduction of oxidative stress indices and the increase of the anti-inflammatory cytokine IL-10. This may down-regulate the pro-inflammatory cytokines INFã and TNFá and inhibit the formation of liver granulomas.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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