HPV genotypes in anal samples from Italian and foreign-born males attending an STD unit in Milan, Italy
Abstract number: P1897
Orlando G., Tanzi E., Fasolo M.M., Beretta R., Amendola A., Bianchi S., Mazza F., Valerio A., Rizzardini G.
Objective: Anal intraepithelial neoplasia (AIN) arising from oncogenic HPV genotypes is increasingly reported, especially in MSM, and is less characterised than cervical disease. The relative distribution and pathogenicity of HPV types has been reported to vary by geographical region arising some questions on the relative efficacy of preventive HPV vaccines. This study wants to evaluate HPV genotypes spectrum in a cohort of males attending an STD unit in MIlan, Italy according to their nationality.
Methods: Anal Pap smear, Hybrid-capture-II HPV test (HC2) and HPV genotyping by RFLP technique performed in Italian (IT) and foreign borne (FB) males recruited from Jan 2003 to Dec 2006 in the STD unit of the II Dept of Infectious Diseases, L Sacco Hospital, Milan (Italy) have been evaluated.
Results: Among 194 IT and 47 FB males recruited, HIV infected patients were 165 (85.05%) and 40 (85.1%) respectively. FB people was younger than Italian (mean age 32.1±6.1 vs 37.4±9.7 p 0.0003) and were mostly from south America (91.5%).
Mean CD4 cells and HIV RNA in HIV infected persons at time of evaluation were comparable. FB patients had a higher rate of abnormal results (ASCUS, LSIL or HSIL) on anal cytology (93.2% vs 80.9% p 0.046). Distribution of High Risk (HR) HPV genotypes on HC2 was comparable as so as results on histology.
HPV-16 was identified in 41.2% of IT and 47.5% of FB and constitutes the 22.8% and 22.6% of all isolates in the 2 groups respectively; HPV type specific prevalence was different between IT and FB people for most of the 29 other different HPV genotypes identified. HPV-13, -40, -44, -54, -64, were specifically isolated among Italian while HPV-45, -66, and -69 only in FB persons. Multiple HPV genotypes infections were demonstrated in 51.7% of Italian and in 68.3% of FB people (p = NS) with a higher prevalence of HR-HPV other than 16/18 in this latter group (26.1% vs 42.5% p 0.041).
Discussion: As already reported for cervical disease, HPV-16 is by far the most common genotype found in anal cytological samples independently from geographycal region while the overall distribution of other HPV genotypes is not overlapping in Italian and Foreign Borne people. These last patients were more likely to be infected with HR-non HPV16/18 types and to harbour more than 1 genotype. The influence of each genotype and the association with the most prevalent HR HPVs on cancer development need to be tested.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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