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Evaluation of anti-leishmanial efficacy by in vivo administration of herbal extract Artemisia auckery on Leishmania major in Balb/c mice

Abstract number: P1872

Sharif M., Nasrolahei M., Daryanii A., Gohardehi S.

Objectives: Cutaneous infection caused by protozoa the genus Leishmania are a major worldwide health problem, with high endemicity in developing countries including Middle East, Africa and Latin America. In the absence of a vaccine, there is an urgent need for effective drugs to replace and supplement those in current use.

Methods: We report in this study the in vivo efficacy of Artemisia auckery herbal extract on the Leishmania major cutaneouse infection in murine model in susceptible Balb/c mice. To carry out this investigation, mice were assigned to the 5 groups (each with 3 mice) as healthy negative control, saline-treated control, glucantim-treated and treated-groups with herbal extract. Experimental Leishmaniasis was initiated by the subcutaneous (s.c.) application of the 2×106 promastigotes of L. major (MRHO/IR/75/ER) into the basal tail of all groups except the healthy negative control group. The development of lesions was determined weekly by measuring the diameters. Inoculations of both Glucantime and herbal extract were carried out by subcutaneous injections once daily for 20 days. At the end of treatment course, all mice were killed humanely by terminal anaestheisa and target tissues including lymph node, spleen and liver from each mouse were removed and weighted and their impression smears were also prepared.

Results: The results indicated that our herbal extract was able to affect on lesion size, its performance and to prevent visceralisation of the parasite. This is the first report indicating visceralisation caused by the cutaneous form of L. major in the Balb/c mice. During this experiment no side effects were observed due to the application of herbal extract in the treated-mice. The impression smears showed a reduction of parasite burdens in spleen, liver and lymph node.

Conclusion: In comparison with glucantim; the present herbal combination was more effective on this murine Leishmaniasis, therefore it could be suggested as a substitute for glucantime in the treatment of Leishmaniasis for human and animal purposes.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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