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Efficacy of amphotericin B and voriconazole against Leishmania tropica with microcapillary culture method

Abstract number: P1869

Bolukbas B., Hokelek M., Beyhan Y.E.

Objectives:Leishmania parasites cause a wide range of human diseases from localised self-healing cutaneous lesions to fatal visceral disease. The causative agent of Cutaneous Leishmaniasis (CL) in southeastern Turkey is mainly Leishmania tropica. A sensitive microcapillary culture method (MCM) was developed for rapid diagnosis of CL by Allahverdiyev et al. The aim of this study is researching the effect of amphotericin B (AMB) and voriconazole (VOR) on Leishmania tropica (MHOM/ TR/EP39) using MCM in order to determine more effective alternatives to antileishmanial drugs which current being used for treatment.

Methods: In this study, the promastigotes these are growth in NNN medium and passaged RPMI-1640 medium (with L-glutamine) buffered with 25 mM HEPES and supplemented with 20% fetal calf serum, are inoculated in mixed solutions prepared with different drug concentrations and medium. Capillary tubes will load with medium and drug dilution. The ends of the capillary tubes were seal with heat-melted candle wax. All inoculated tubes incubated at +26°C for 72 hours. End of time, the capillary tubes are cut off ends and promastigotes are counted on Thoma chamber. The 50% inhibitory concentrations (IC50) are calculated.

Results: The IC50 values for AMB and VOR were determined that 0.1475 mg/ml, 4.682 mg/ml, respectively.

Conclusion: In conclusion, it was found that AMB and VOR have got inhibitory effects of L. tropica promastigotes in vitro. IC50 values of VOR more than AMB, but due to their too few toxic effects than many antileishmanial drugs, they may be alternative treatments in future. In addition, the MCM has been used for the first time on drug investigations, which was seen to be valuable for in vitro drug tests of Leishmania as well as routine diagnosis of leishmaniasis. Further studies are needed to develop these results with in vivo CL animal models.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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