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Identification of Leishmania species isolated from human cutaneous leishmaniasis in Gonbad-e Qabus using a PCR method

Abstract number: P1868

Mesgarian F., Rahbarian N., Mahmoudi Rad M., Hajaran H., Shahbazi F., Mesgarian Z., Taghipour N.

Objectives: To determine the epidemiological status of the cutaneous leishmaniasis outbreak, isolation and identification of the parasite using a PCR method in Gonbad-e Qabus County, north Iran, during 2006–2007.

Methods: Data was collected on the prevalence of scars and ulcers over a period of 16 months among 6990 inhabitants of five villages around Gonbad-e Qabus County. Cultured promastigotes were identified using PCR technique. ITS1 and ITS2 of Non Coding Transcribed region at ribosomal DNA of 46 Leishmania isolates were amplified and the PCR products were separated by electrophoresis in 2% agarose gel (200 mA, 140 V), visualised by staining with ethidium bromide, and photographed.

Results: Among 6990 inhabitants of 5 villages, 62.9% had scars and 1.5% had active lesions. Individuals 11 to 20 years of age were the most highly infected age group. All the parasite isolates were Leishmania major.

Conclusion: According to this study, cutaneous leishmaniasis due to Leishmania major is endemic in Gonbad-e Qabus County, north Iran.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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