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Mycobacterium avium ssp. paratuberculosis and cytokine response in inflammatory bowel diseases

Abstract number: P1817

Szkaradkiewicz A., Marciniak R., Chudzicka-Strugala I., Wasilewska A., Drews M., Majewski P., Zwozdziak B., Karpinski T.

Objectives: The studies aimed at analysis of manifestation of Mycobacterium avium ssp. paratuberculosis (MAP) infections as related to secretion of selected cytokines (TNF-alpha, IL-1beta, IL-8 and IL-10) in patients with ulcerative colitis (UC) and Crohns disease (CD).

Methods: The studies were performed on three groups of adult patients: group 1 included 20 persons with UC, group 2 consisted of 12 patients with CD, group 3 (control group) was formed by 15 healthy adult volunteers, in whom routine colonoscopy was performed.

Material for the studies consisted of sera originating from the patients of all the three groups and intestinal biopsies from patients of group 1 and group 2. In the sera levels of TNF-alpha, IL-1beta, IL-8 and IL-10 were estimated, employing high sensitivity ELISA kits (Quantikine HS, R and D Systems) for estimation of TNF-alpha, IL-1beta and IL-10 and ELISA kit (Quantikine, R and D Systems) for estimation of IL-8. DNA was isolated from tissue material (QIAamp DNA Mini Kit, Qiagen) and tested for the presence MAP DNA using IS900-specific nested PCR test (Institut Pourquier, France).

Results: In group 1 with UC MAP DNA was detected in 5 (25%) patients. Mean concentrations of studied cytokines amounted to: TNF-alpha – 1.64 pg/ml, IL-1beta – 1.44 pg/ml, IL-8 – 49.74 pg/ml, IL-10 – 2.97 pg/ml.

In group 2 with CD MAP DNA was detected in 8 (66.6%) patients. Man concentrations of studied cytokines were as follows: TNF-alpha – 3.12 pg/ml, IL-1beta – 0.77 pg/ml, IL-8 – 53.70 pg/ml, IL-10 – 2.59 pg/ml.

In statistical analysis MAP DNA was found to manifest significantly more frequently in patients with CD. In parallel, independently of the presence/absence of MAP infection, serum concentrations of TNF-alpha, IL-1beta and IL-8 in patients with inflammatory bowel diseases were elevated as compared to those in the control group while serum levels of IL-10 were the same in all the studied groups.

Conclusion: Inadequate secretion of IL-10 in parallel to the augmented secretion of pro-inflammatory cytokines (TNF-alpha, IL-1beta and IL-8) may play a principal role in development of inflammatory bowel diseases, while the dominating stimulant of pro-inflammatory cytokines production in CD seems to involve MAP.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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