In vitro selection of probiotic multispecies against Clostridium difficile 027

Abstract number: P1784

Veenendaal D., Dijkstra M., Timmerman H.

Clostridium difficile (CD) ribotype 027 is an emerging disease causing severe outbreaks of diarrhoea in hospitals as wel as the community in Europe, North America, Canada and Japan. Treatment options for C. difficile associated diarrhoea (CDAD) consists of preventive and treatment measures i.e. controlled use of antibiotics, hygiene measures and treatment with vancomycin or metronidazol. Still mortality (>10%) and mutiple relapses (>20%) are persisting challenges to overcome. Sofar probiotics have no place in the treatment or prevention of 027 associated CDAD due tot lack of convincing clinical studies and severe side effects e.g. the yeast Saccharomyces boulardii. Most of the clinical studies are carried out with only one or a limited numer of probiotic strains. Still many lactic acid producing probiotic species like lactobacilli, bifidobacteria and lactococci have found to inhibit CD. We studied the inhibitory potential of 28 probiotic species against 6 CD 027 and non-027 strains in solid as well as liquid culture. Whereas all CD strains were equally effected, substantial differences were found between probiotic strains even within one specie. Probiotic whole cell cultures (cells + extracellular components) were found to be significantly more effective than extracellular components only. Remarkably no correlation was found between CD growth inhibition and pH nor the natural production of lactic acid. Probiotic multispecies were found more effective against CD 027 in liquid co-cultures as compared tot singlespecies. Still single probiotic species were found to inhibit toxin production despite a failure in reducing growth of CD 027. Co-culture in solid as well as liquid media may be very helpfull in selecting potential combinations of probiotic multispecies for clinical treatment or preventive studies against C. difficile.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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