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Is Helicobacter pylori the only aetiologic factor involved in the pathogenesis of gastric cancer among Iranian population?

Abstract number: P1696

Mohajerani N., Talebkhan Y., Esmaeili M., Bababeik M., Oghalaie A., Nahvijoo A., Eshagh Hosseini M., Mohagheghi M.A., Abdirad A., Mohammadi M.

Objectives:Helicobacter pylori has been documented as an aetiological agent in gastric cancer (GC) development. Hp immuno-reactive proteins and its main virulence factors, CagA and VacA, are implicated in the pathogenesis of related diseases. On the other hand, host genetic susceptibility and gastric hormones have been proposed as important indicators in screening approaches for GC development. This study has aimed to explore the aetiologic role of Hp, its antigens and their association with the other mentioned factors in developing GC among Iranian patients.

Methods: Hp status was determined by three gold standard tests. 309 patients referring for endoscopy and 198 GC cases were included in this study. Immunoblotting, ELISA and gene-specific PCRs were performed on collected samples.

Results: Hp infection was detected in 78.8% of all subjects composed of 80.3% of dyspeptic (non GC) subjects and 76.5% of GC patients. Sero-reactivity toward Hp was detected in 91–93% of both studied groups. vacA s1m1 and s2m2 genotypes were most prevalent in GC and non GC patients, respectively. Serological data revealed that unlike cagA genotyping which revealed no association with disease, the presence of anti CagA antibody can be a predictive marker in GC development. Antibody response towards Hp LMW antigens especially 35kDa and 37kDa proteins are differentially prevalent in two studied groups. Histo-pathological data revealed that there is no significant association between the type of tumour and Hp positivity. Host interleukin-1 genotyping indicated that there is a higher prevalence of Hp infection in subjects with L/L genotype of IL-1RN irrespective of the clinical picture while Hp negative subjects possess mostly the 2/2 allele. In both Hp positive and negative groups the mean and standard deviation of pepsinogen I and II levels were similar.

Conclusion: This study showed that although the absolute majority of the studied subjects were infected with Hp, genotyping of isolated Hp strains and serological analysis can be appropriate screening approaches for high risk populations. Evaluation of host susceptibility factors revealed that IL-1 beta and its receptor antagonist may be involved in GC pathogenesis irrespective of Hp infection. The genetic background of the normal population, however, should be investigated before making a firm conclusion about this contribution to GC development.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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