Use of MIRU-VNTR typing to differentiate Mycobacterium tuberculosis isolates from Bulgaria and comparison with IS6110-RFLP typing and spoligotyping
Abstract number: P1678
Valcheva V., Mokrousov I., Narvskaya O., Rastogi N., Markova N.
Objectives: To assess the discriminatory power of different molecular typing methods (IS6110-RFLP, Spoligotyping, MIRU-VNTR) used alone and in different combinations to evaluate the most appropriate combination and usefulness of VNTRs for molecular-epidemiological studies of M. tuberculosis in Bulgaria.
Methods: IS6110-RFLP, Spoligotyping, MIRU-VNTR were used for strain differentiation.
Results: 73 M. tuberculosis strains were subdivided into 68 types (21-loci and 15-loci MIRU-VNTR scheme), 59 types (12-loci MIRU-VNTR scheme), 51 types (IS6110-RFLP) and 31 types (spoligotyping). Among 21 tested VNTR loci, we selected 5 loci that allowed to achieve sufficiently high discrimination (52 types). The Hunter-Gaston discriminatory index was as follows: Spoligotyping (0.939), IS6110-RFLP typing (0.983), 12-loci MIRU-VNTR typing (0.992), 5-loci MIRU-VNTR typing (0.988), 21-loci and 15-loci MIRU-VNTR typing (0.997).
Conclusion: We conclude that both MIRU-VNTR typing and spoligotyping methods are rapid, highly reliable and discriminatory methods for epidemiological typing of M. tuberculosis; in the Bulgarian setting, MIRU-VNTR typing is the epidemiological method of choice for the near future.
Acknowledgments: This work was supported by NATO grant SFP 982319 "Detect drug-resistant TB".
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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