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Biodiversity of UTI pathogens in diabetics

Abstract number: P1616

Saleem M.

Introduction: Urinary tract infection (UTI) is a significant health problem affecting people worldwide. Women are especially prone to UTI for reasons which are poorly understood, common factor known is the shortness of urethra which contributes to the easy passage of uropathogens into the bladder. Any abnormality in the urinary tract that obstructs the flow of the urine sets a stage for infection.

Diabetic patients have a higher risk of acquiring UTI because of the changes in the immune system. Any disorder that suppresses the immune system like diabetes raises a risk of UTI.Some of them are potentially lethal like mucormycosis which never affects people without diabetes. Urinary tract is also a cause of bactraemia among patients with diabetes, it has been established that uropathogens like E. coli grow better in urine after the addition of glucose in various levels. Many people with diabetes have dysfunctional bladders which contract poorly & hence allows the urine to remain stagnant for longer periods thus providing luxorious ponds for bacteria to grow in.

The current study is based on:

–The incidence of UTI in diabetic patients.

–To detect most prevalent strains of uropathogens.

–To develop link between diabetes and lower socio economic group.

–Risk factors associated with UTI in diabetic patients.

–Screening of MDR strains.

Methods: The prospective study involved collection of urine samples from diabetic patients belonging to higher & lower socioeconomic group. Urinalysis and culturing methods were followed to isolate uropathogens from the samples collected. Confirmation of possible UTI was achieved through viable colony counts in CFU/ml of the specimen.

Analysis of C-Reactive protein was performed in patients with diabetes, without diabetes, and in patients with diabetes but no UTI.

Clinically significant uropathogens were checked for resistance hence isolation of some MDR strains was established.

Isolation of plasmids was performed in these strains to detect specific mutations associated with diabetes induced UTI.

Experiments were also carried out for the estimation of iron in diabetic blood samples so as to corelate the association of these factors with UTI.

Results: 500 patients were evaluated for the study, and the results indicated 46.4% people had diabetes. Enterococcus faecalis 25%, Pseudomonas aeruginosa 15.94%, E. coli 7.4%, Klebsiella 3% & Staphylococcus 10.34% were found to be frequently encountered UTI pathogens.

Conclusion: Prevalence of UTI is higher in diabetic patients.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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