Molecular epidemiology and disease severity of respiratory syncytial virus in relation to other potential pathogens in children hospitalised with acute respiratory infection in Jordan
Abstract number: P1603
Kaplan N.M., Dove W., Abd-Eldayem S.A., Abu-Zeid A.F., Shamoon H.E., Hart C.A.
Objectives: To examine the molecular epidemiology of human respiratory syncytial virus (HRSV) in Jordan. To compare the disease severity of HRSV subgroups A and B and their associated genotypes in hospitalised Jordanian children with other potential respiratory pathogens.
Methods: Between December 2003 and May 2004, a total of 326 Jordanian children younger than 5 years of age and hospitalised with acute respiratory tract infections were recruited to the study. Total RNA and DNA were extracted from the collected nasopharyngeal aspirates. HRSV was detected by reverse transcription polymerase chain reaction (RT-PCR) using specific primers that target the nucleocapsid gene to produce a 278-bp amplicons. HRSV-positive strains were classified into subgroup A and B by restriction fragment length polymorphism analysis. Other respiratory pathogens were detected by RT-PCR and PCR according to previously published protocols.
Results: A total of 254 patients had at least one potential pathogen detected and 106 had mixed infections. 67 out of 140 HRSV-positive patients were co-infected. Significantly (p < 0.0005), more HRSV-infected children had severe disease compared to those uninfected with HRSV. Severe HRSV infections were significantly more likely to occur in those under 6 months of age (p < 0.01). There was no significant difference between the individual HRSV genotypes as potential causes of severe respiratory tract disease.
Conclusion: HRSV was the most frequent cause of severe acute respiratory infection in young children in Jordan. HRSV subgroup A was more predominant than subgroup B. Only four of the six previously described N genotypes were found among HRSV strains. Further studies over longer periods of time are warranted to better determine the epidemiology and disease severity of HRSV in Jordan.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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