Interest of a new technique of DNA chips for detection of respiratory viruses
Abstract number: P1601
Causse M., Garcia A., Gutierrez J., Casal M.
Objective: The aim of this study is to evaluate a multiplex RT-PCR combined with a new DNA-Chip hybrisation, comparing it to indirect immunofluorescence for detecting respiratory viruses.
Methods: We used 114 nasopharyngeal washing samples from our Hospital, each of them corresponding to different patients between November 2006 and January 2007. They were all between 2 months and 5 years old.
All the samples were performed: Indirect immunofluorescence (following protocol by Bartells©) and DNA Chips (Amersham Biosciences) for Respiratory Syncitial Virus, Adenovirus, Influenzavirus A and B and Parainfluenzavirus I, II and III, and Inmunochromatography for RSV (Binax©, Marnes la Coquette, France).
Those samples which resulted positive by, at least, two techniques, were considered true positives. The same was considered for true negatives.
Results: See table 1.
Conclusions: DNA chips avoid subjectivity in the interpretation of Immunofluorescence and are not affected by the tester's experience, as results reading is automated (BCS AiM Reader). However, it is a laborious technique with a higher duration than Immunofluorescence (about 6 hours vs the 1 and half which takes Immunofluorescence).
We obtained a very similar sensitivity and NPV values with both techniques close to 95%, but a better statistically significant (p < 0.05) specifity and PPV with DNA-chip.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
|Back to top|