Interest of a new technique of DNA chips for detection of respiratory viruses

Abstract number: P1601

Causse M., Garcia A., Gutierrez J., Casal M.

Objective: The aim of this study is to evaluate a multiplex RT-PCR combined with a new DNA-Chip hybrisation, comparing it to indirect immunofluorescence for detecting respiratory viruses.

Methods: We used 114 nasopharyngeal washing samples from our Hospital, each of them corresponding to different patients between November 2006 and January 2007. They were all between 2 months and 5 years old.

All the samples were performed: Indirect immunofluorescence (following protocol by Bartells©) and DNA Chips (Amersham Biosciences) for Respiratory Syncitial Virus, Adenovirus, Influenzavirus A and B and Parainfluenzavirus I, II and III, and Inmunochromatography for RSV (Binax©, Marnes la Coquette, France).

Those samples which resulted positive by, at least, two techniques, were considered true positives. The same was considered for true negatives.

Results: See table 1.

Conclusions: DNA chips avoid subjectivity in the interpretation of Immunofluorescence and are not affected by the tester's experience, as results reading is automated (BCS AiM Reader). However, it is a laborious technique with a higher duration than Immunofluorescence (about 6 hours vs the 1 and half which takes Immunofluorescence).

We obtained a very similar sensitivity and NPV values with both techniques close to 95%, but a better statistically significant (p < 0.05) specifity and PPV with DNA-chip.

Table 1.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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