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Microbiological effects of a triclosan dentifrice and tongue cleaner in the treatment of oral malodour

Abstract number: P1544

Zambon J., Moses P., Clark B., Haraszthy V., Sreenivasan P., DeVizio W.

Objectives: Global surveys indicate that oral malodour (halitosis) is a widespread condition affecting almost 50% of people at sometime. Oral malodour is frequently associated with bacteria on the dorsal surface of the tongue. The large surface area and unique anatomy of the dorsal tongue including fissures and papillae facilitate the growth of halitosis-associated microorganisms. This study assessed the effect of treatment for oral malodour on the microflora of the dorsal surface of the tongue.

Methods: Adults were screened for oral malodour and were selected for participation in the study if they exhibited organoleptic scores leqslant R: less-than-or-eq, slant3 (scale 0–5) as determined by a trained examiner and mouth air sulfur levels leqslant R: less-than-or-eq, slant250 ppb as determined by portable sulfide detector. 15 adults were enrolled in the study after informed consent. Each subject was examined at baseline and after 28 days for; (1) organoleptic assessment; (2) mouth air sulfur levels; (3) tongue coating (scale 0–3); and, (4) tongue microorganisms. A 2 cm2 area was sampled from the dorsal surface of the tongue. Bacterial numbers were assessed by microscopy and the presence of 22 bacterial species/groups was assayed by dot-blot using DNA probes. Following the baseline examination, the subjects were instructed to perform oral hygiene with a triclosan-containing dentifrice and to use a toothbrush with a tongue cleaner.

Results: From the panel of halitosis-associated target bacteria, we observed large numbers of Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia/Prevotella loescheii, Prevotella melaninogenica, Solobacterium moorei and Streptococcus sp. at baseline. After 28 days, all subjects showed clinical improvement as determined by significant reductions in organoleptic scores and mouth air sulfur levels compared to baseline (p < 0.0001). Microscopic analysis showed a greater than 70% reduction in the numbers of microorganisms (p < 0.001) while dot-blot assays showed significant reductions in Enterococcus faecalis (p < 0.003), Neisseria sp. (p < 0.008), P. micros (p < 0.0007), P. melaninogenica (p < 0.02), P. gingivalis (p < 0.0001), S. moorei (p < 0.04), and Streptococci sp (p < 0.03) from the dorsal tongue surface.

Conclusions: All subjects with oral malodour demonstrated significant improvements in both clinical and microbiological parameters following use of the triclosan-containing dentifrice and toothbrush with a tongue cleaner.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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