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High Panton-Valentine leukocidin prevalence among meticillin-sensitive Staphylococcus aureus from Yogyakarta, Indonesia

Abstract number: P1448

Deurenberg R.H., Beisser P.S., Visschers M.J., Driessen C., Utomo T., Stobberingh E.E.

Objectives: During the last decade, community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) has emerged. CA-MRSA is characterised by staphylococcal cassette chromosome mec (SCCmec) type IV or V, several continent-specific genetic backgrounds, and the presence of Panton-Valentine leukocidin (PVL). CA-MRSA clones could emerge through the transfer of the highly mobile SCCmec type IV element into PVL-positive meticillin-sensitive S. aureus (MSSA) with a genetic background common to CA-MRSA. Therefore, studies are needed to investigate the prevalence of PVL among MSSA isolates.

Methods: During September and October 2006, 440 nasal swabs were taken from outpatients (n = 320) and their companions (n = 320) at the Department of Ear, Nose and Throat at the Dr. Sardjito Academic Hospital in Yogyakarta, Indonesia. The swabs were send to The Netherlands for isolating and identification of S. aureus. These were characterised with spa typing and the algorithm based upon repeat pattern (BURP). The prevalence of the virulence factors collagen adhesion (CNA), PVL and toxic shock syndrome toxin 1 (TSST-1) was investigated.

Results: From 62 individuals (14%), including 39 outpatients (18%) and 23 companions (11%), S. aureus was isolated by standard microbiological methods. Typing revealed 37 spa types (of which 9 were new spa types), and 20 spa types were clustered into 6 spa-clonal complexes (spa-CCs) (42 isolates). The remaining isolates could not be classified into spa-CCs. Up to 69% of these isolates were associated with a genetic background common to the endemic MRSA clones, e.g. clonal complex (CC)1, CC8, and CC45. The remaining isolates were associated with CC12, CC72, and CC121. The prevalence of CNA, PVL and TSST-1 was 74%, 26% and 16% respectively; the presence of PVL was strongly associated with CC1.

Conclusions:

1There was a low S. aureus prevalence (16%) among the population investigated, compared to the normal prevalence of between 30% and 50%.

2No MRSA isolates were observed in the population.

3A relatively high PVL-prevalence among MSSA isolates with a genetic background common to a virulent and epidemic CA-MRSA clone (CC1/USA400) was observed.

4CA-MRSA clones might emerge through the integration of SCCmec type IV into PVL-positive MSSA with a genetic background common to CA-MRSA.

5Studies are necessary to monitor the possible emergence of CA-MRSA clones, and limit their subsequent dissemination.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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