Distribution of Nocardia species in clinical specimens in Greece
Abstract number: P1400
Klapsa D., Auzou M., Bartziali S., Vlahaki E., Vergi M., Koutsia-Carouzou C., Malamou-Lada E., Leclercq R., Petinaki E.
Objectives: The aim of this study was to assess the species distribution of a large number of Nocardia isolates in Greece and to propose a rapid identification test that may be helpful to identify the species most commonly encountered in clinical material.
Methods: A total of 100 Nocardia spp. isolates, recovered from clinically significant specimens, were identified using API-ZYM, according to the procedures of manufacturer and a molecular method based on hsp65 gene. The API-ZYM system, including 19 enzymes (alkaline phosphatase, esterase lipase, leucine arylamidase, acid phosphatase, phosphohydrolase, alpha-glucosidase, beta-glucosidase. valine arylamidase, cystine arylamidase, trypsin, chymotrypsin, alpha-galactosidase, beta-glucoronidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase, alpha-fucosidase, beta-galactosidase, ONPG) determined the enzyme profile of Nocardia species. On the other hand, DNA extracted from Nocardia species was amplified using as target the hsp65 gene. PCR products were then, purified and sequenced, while, identification to the species level was done after comparison with all sequences available at the BLAST Genome.
Results: Results obtained by API-ZYM system failed to discriminate the isolates to the species level. On the other hand, molecular method identified twenty isolates as N. flavorosea, thirty-four as N. cyriacigeorgica, twenty-six as N. nova, two as N. veterana, twenty-four as N. farcinica, two as N. abscessus, and two as N. asteroides.
Conclusions: Genus-specific hsp65 gene sequencing can be a rapid and reliable adjunct in the diagnosis of Nocardia to the genus and species level.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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