Fungal colonisation of gastro-intestinal tract, anti-Saccharomyces cerevisiae antibodies and anti-mannan Candida antibodies in patients with inflammatory bowel disease and irritable bowel syndrome
Abstract number: P1347
Semianów-Wejchert J., Nawrot U., Ksiadzyna D., Wlodarczyk K., Bartnik A., Annabhani A.
Introduction: A search for serologic markers useful in diagnostics, including differential diagnostics of inflammatory bowel disease (IBD) continues. The idea that microorganisms play a role in the aetiology of IBD has gained ground considerably in recent years. Antibodies against mannans of Saccharomyces cerevisiae (ASCA) were observed in Crohn's disease in 1988. It is said that anti-mannan Candida spp. antibodies (AMCA) may be useful in diagnostics of often latent fungal infections. Therapy with antibiotics as well as immunosuppressive treatment favour fungal infections especially in the presence of intestinal mucosa lesions.
The aim: to assess the level of ASCA and AMCA and the incidence and intensity of fungal colonisation of gastrointestinal tract in IBD patients in comparison with irritable bowel syndrom (IBS) patients.
Materials and Methods: 42 patients suffering from ulcerative colitis (UC) and 26 patients with Crohn's disease (CD) as well as 19 IBS patients were subjected to the study. The level of antibodies was assessed with ELISA. The quantitive and qualitative fungal cultures of the stool were performed in all the subjects using Sabouraud agar plates supplemented with chloramphenicol and Chromagar-Candida ID-32-Biomerieux test.
Results: The results were obtained as shown in the table.
1ASCA were found in 58.3% of CD, 33.3% of IBS and 6.7% of UC patients.
2The level of ASCA in CD patients was significantly higher then in UC and IBS patients (p = 0.002 and p = 0.003, respectively).
3The increased level of AMCA and significant fungal colonisation were observed more often in IBS patients then in IBD patients.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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