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The evaluation of three pro-inflammatory cytokines

Abstract number: P1337

Sharifat Salmani A., Siadat S.D., Ahmadi H., Norouzian D., Tabaraiee B., Alimagham M., Farahmand S., Kheirandish M., Zangeneh M., Behzadiannejad Q.

Objective: Smooth Lipopolysaccharide (S-LPS) is the most antigenic structure expressed on the surface of Brucella smooth strains which promotes the innate and adaptive immune responses. LPS-induced innate immune responses are involved in the proinflammatory reactions and the release of proinflammatory cytokines, such as TNF-a, IL-1Beta and IL-6. The controlled increase of proinflammatory responses would be achieved by the application of adjuvants and studied as a method to induce the innate immune responses against the LPS of Brucella. Outer membrane vesicle of Neisseria meningitidis is one of the newly studied adjuvants with microbial origin and Several studies carried-out about the probability of Neisseria meningitidis OMV application as an adjuvant. We have analysed the induction of three proinflammatory cytokines(TNF-a, IL-1b and IL-6)in immunised animal models with extracted S-LPS of B. abortus S99 complexed with OMV of N.meningitids, as an adjuvant.

Methods: The S-LPS of Brucella was extracted by hot phenol-water method. Then extracted S-LPS injected to mice in two different patterns, 1: B. abortus S-LPS 2: B. abortus S-LPS-N. meningitidis OMV (OMV as a probable potent adjuvant) noncovalent complex. After injections, animals were bled in 7, 14, 21 and 28'th and Finally sera analysed to detect the level of TNF-alfa, IL-1b and IL-6 by ELISA method.

Results: IL-6 was the first detected cytokine one week after the first injection. Peak amount of IL-6 detected two weeks after the first injection and there was not a significant difference between IL-6 amounts in LPS and LPS-OMV immunised groups. TNF-a and IL-1b were not detected in serum of S-LPS-immunised animals after the injections but interestingly both of these two cytokines were detected in the serum of animal inoculated with the S-LPS-OMV complex. The peak amounts of IL-1b and TNF-a were detected three weeks after the injection.

Conclusion: OMV can efficiently induce the production of IL-1b and TNF-a in the studied animal models. Since IL-1b and TNF-alfa are cytokines with innate cellular immunity sources and innate immunity is an important primary barrier against Brucella infection, we can conclude that OMV could be applied as an effective adjuvant in the candidate vaccines to elicit a more potent cell mediated innate immune response, in parallel with the augment of specific humoral and cellular responses against Brucella LPS.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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