Intestinal Oxalobacter formigenes colonisation in children with calcium oxalate urolithiasis. A study from southeastern Poland.
Abstract number: P1319
Sikora P., Niedzwiadek J., Mazur E., Niedzwiadek A., Zajaczkowska M., Koziol-Montewka M.
Objective: Oxalobacter formigenes is an obligate anaerobic Gram-negative bacterium colonising the colon of vertebrates, including humans. This organism utilises oxalate as the only source of carbon and therefore participates in oxalic acid homeostasis. In adults, it was suggested that the lack of colonisation with O. formigenes may lead to the intestinal oxalate hyperabsorption and to calcium oxalate urolithiasis (CaOxU) as a consequence. To our knowledge this problem was not investigated in paediatric population. Hence, the aim of our study was the assessment of O. formigenes colonisation rate in children with CaOxU.
Methods: DNA was extracted from the frozen faecal samples of 84 children and adolescents (41 boys, 43 girls) aged 4.917.6 years with CaOxU and 63 healthy, age- and gender matched subjects. Identification of O. formigenes was performed by polymerase chain reaction (PCR) using genus specific primers, according to Sidhu et al. (JCM 1999, 37, 15031509).
Results: O. formigenes was found in 23.8% (20/84) of patients with CaOxU and in 20.6% (13/63) of healthy controls.
Conclusions: In contrast to adults, we did not find any significant difference in O. formigenes colonisation rate between individuals with or without urolithiasis. However, an absence of O. formigenes in majority of children from both groups requires further explanation. It could be caused by an overuse of antibiotics in our region. Also, we can not exclude that extreme variability in the O. formigenes content of stool samples among individuals may be responsible for the low rate of O. formigenes identification in both examined groups.
The research was supported by the Polish State Committee for Scientific Research (KBN grant 2P05D 117 26).
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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