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Identification and genotyping of uropathogenic Escherichia coli strains isolated from patients with urinary tract infection based on papC and papG genes using PCR

Abstract number: P1297

Zamanzad B.

Introduction:Escherichia coli (E. coli) is the most common aetiologic agent of Urinary tract infections (UTIs). The most important virulence factor in Uropathogenic E. coli (UPEC) strains is P fimbriae. P fimbriae is coded by pap(pyelonephritis associated pili) operon. PapG is the most common adhesin in this fimbriae and papC is an important gene in this operon. Fimbriated uropathogenic strains of E. coli have an important role in causing UTI especially upper tract infections. In this study prevalence of pap operon (papC and papG genes) in E. coli strains isolated from nosocomial UTIs were evaluated.

Methods: A total of 182 E. coli isolates cultured from patients with different clinical forms of UTI referred to Shahre-kord teaching hospital were included. DNA of bacterial isolates was extracted by boiling method. PCR method was performed for presence of papC (pap operon) and papG genes. Also, genotyping of papG (pap operon) positive isolates were performed using multiplex PCR method.

Results: The prevalence of pap operon in isolates was 36.2%. Genotyping of P fimbriated isolates for papG gene by multiplex PCR method showed the prevalence of class 2 and class3 of papG gene, 23.1% and 6.6% respectively. None of the isolates had class 1 genotype. Furthermore, papG class 2 was predominant in patients with pyelonephritis and papG class 3 was predominant in ones with cystitis. In this study, no relationship between the presence of P fimbriae and sex of the patients was detected.

Conclusion: PapC (and papG)) genes have important roles in pathogenesis of fimbriated uropathogenic strains of E. coli. We recommend detection and genotyping of these genes in complicated UTIs in hospitalised patients enabling specific diagnosis and treatment of nosocomial infections with urinary tract origin.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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