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Distribution of virulence factors in E. coli strains isolated from women with urinary tract infection by PCR

Abstract number: P1296

Alepopoulou E., Panopoulou M., Konstantinidou E., Konstantinidis T., Kalpini D., Kartali S.

Objective:E. coli is the most common cause of urinary tract infections (UTI). A variety of urovirulence factors are overrepresent in uropathogenic E. coli, including fimpriae and adhesins, cytotoxins such as a-haemolysin and cytotoxic necrotising factor 1, aerobacin-mediated iron uptake system and others. The dominance of strains expressing multiple urovirulence factors may further increase the risk for development of UTI specifically in women. In the present study, that was comparables in University hospital of Alexandroupolis in northern Greece, we analysed the distribution of genes pap, hlyA, cnf1, aer, sfa and afa in E. coli strains isolated from women with UTI.

Methods: A total of 103 strains E. coli were isolated from 103 women 18 to 83 years old with UTI. The laboratory criterion for acute E. coli UTI was the presence of positive culture on MC agar response with at least 105 CFU/ml. Multiplex PCR was performed to detect urovirulence genes pap, hlyA, cnf1, aer, sfa, afa in E. coli strains. Amplification products 336bp, 1177 bp, 498 bp, 602 bp, 410 bp and 750 bp respectively were electophoresed in 2% agarose gel and visualised by ethidium bromide staining.

Result: Seventy seven (75%) of the 103 strains E. coli were found to carry at least one urovirulence gene. Frequencies of genes pap, hlyA, CNF, aer sfa, afa in total 103 isolates were estimated as 45%, 20%, 25%, 47%, 27% and 6% respectively. The data indicated that 2%, 8%, 14%, 17%, 27% and 6% isolated carried six, five, four, three, two and one virulence factor respectively. However no virulence factors were found in 26 (25%) isolates.

Conclusion: The E. coli strains expressing multiple virulence factors comprise frequent cause developing UTI. The results showed that 75% of E. coli isolates carried at least one virulence factor. The occurrence of pap and aer was higher compared with the rest of others genes. In contrast, afa gene was carried in only 6 strains. Also in the present study was demonstrated that the multiplex PCR is an efficient tool for rapid detection and identification of UTI virulence factors in E. coli isolates.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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