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Presence of ESBL-producing Enterobacteriaceae in the stools of outpatients with intra-abdominal infections requiring surgery

Abstract number: P1121

Algarate S., Seral C., Llaneza M.E., Pardos M., Chacón R., Salinas J.C., Castillo F.J.

Objective: Bowel flora may provide a substantial reservoir of resistant microorganisms and precedes intra-abdominal infections. Their susceptibility patterns may provide useful information for empiric treatment. We determinate the flora that colonises the bowel, the incidence of ESBL-producing and/or carbapenem-resistant Enterobacteriaceae (Eb) and the colonisation by non-fermenters Gram-negative bacilli (NF-BGN) in the stools of outpatients with intra-abdominal infections requiring surgery in 71 selected patients from 2005 to 2007.

Methods: Rectal swabs were collected at baseline and inoculated on MacConkey agar plates (MC), MC supplemented with cefotaxime (CTX) 1 mg/L, MC with ceftazidime (CAZ) 1 mg/l and in modified CCDA containing cefoperazone 32 mg/L. Bacterial colonies were identified from each plate using routine laboratory techniques. Eb growing in antibiotic supplemented media were identified and screened for ESBL production by the double-disk synergy test. Confirmation was performed by E-test with CTX/CTX-clavulanic acid (CA), CAZ/CAZ-CA and cefepime (FEP)/FEP-CA.

Results: Rectal swabs were obtained at baseline in 71 selected patients. In 69 of 71 patients (table) growed Eb and abscence of G- faecal flora in two patients. Nine (12.7%) patients harboured an ESBL-producing Eb (4 E. coli CTXR-CAZS-FEPS, 2 E. coli CTXR-CAZR-FEPS, 2 E. coli CTXR-CAZS-FEPR and 1 C. freundii CTXR-CAZS-FEPS) and 9 patients were colonised by NF-BGN (12.7%).

Table: Species of Enterobacteriaceae identified and susceptibility to some b-lactams

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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