Triggering receptor expressed on myeloid cells (TREM-1) gene expression in critically ill patients
Abstract number: P1068
Kotsaki A., Vaki I., Dimopoulou I., Kranidioti H., Orfanos S., Pelekanou A., Giamarellos-Bourboulis E., Armaganidis A.
Objectives: Triggering receptor expressed on myeloid cells (TREM-1) is a recently discovered receptor on monocytes and neutrophils. When stimulated, TREM-1 amplifies the toll-like receptor(TLR)-initiated response to invading pathogens allowing the secretion of pro-inflammatory chemokines and cytokines. Our aim was to estimate TREM-1 mRNA levels in critically ill patients.
Methods: Ten ml of heparinised venus blood were collected upon admission from 14 critically ill patients, five with systemic inflammatory response syndrome (SIRS) and nine with severe sepsis/septic shock (ACCP/SCCM criteria). Peripheral blood mononuclear cells were isolated. RNA was extracted after trizol and chloroform treatment and cDNA was synthesised. Expression of mTREM-1 was estimated by real time PCR against the expression of reference gene of beta-2-microglobulin. RNA isolated from PBMCs of healthy donor was applied, as control.
Results: Quantification of mRNA transcription levels using PFAFL equation showed that the levels of mRNA were higher in patients with SIRS (range 2.9×1031.5×107, median 1×106 copies) compared to patients with severe sepsis (range 13.33×106, median 1×103 copies).
Conclusions: Although former results using flow cytometry analysis has showed that TREM-1 receptor is up regulated in sepsis, pronounced TREM-1 gene expression was found in SIRS compared to severe sepsis/septic shock. These results render the hypothesis that when TREM-1 receptors reach a critical amount to amplifie the inflammatory response, internal signals stop further mTREM transcription.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
|Back to top|