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Relationships between urinary IL-18, MMP-9 and BMP-7 concentration and renal lesion in HIV-1 infected patients

Abstract number: P1000

Dabrowska M.M., Wiercinska-Drapalo A., Siwak E., Kranc R., Flisiak R.

Objectives: HIV infection of glomerular endothelial and mesangial cells can cause progressive renal lesion. IL-18 and MMP-9 which are overexpressed during an HIV-1 infection can stimulate proinflammatory response in the kidney, which can be counterbalanced by increased production of BMP-7.

Methods: A cross-sectional study was performed in 76 HIV (+) patients (57 male, 19 female, aged from 20 to 59 years, 58 on HAART) and 17 HIV (-) volunteers. Plasma HIV-1 RNA was evaluated using the Amplicor system. Urinary IL-18, MMP-9 and BMP-7 concentration was measured by ELISA (Ray Bio and R&D Systems respectively). Creatine clearance was calculated by using of Cockroft-Gault formula. In statistical analyses U Mann-Whitney and Spearman correlation tests were used.

Results: We observed the increase in urinary MMP-9 and the decrease in urinary IL-18 in HIV (+) patients in comparison to control group (6.09±1.39 v. 4.5±0.94 ng/ml, p<0.05 and 15.28±2.54 v. 42.66±11.02 pg/ml, p<0.05). There were no significant differences in urinary BMP-7 regarding to HIV (-) group (2.61±0.54 v. 3.16±0.68 pg/ml, p = 0.09). No significant correlation was found beetwen CDC classification and urinary IL-18, MMP-9 and BMP-7 concentration. The mean urinary MMP-9 concentration observed in patients undergoing HAART was significantly lower than in patients without ARV treatment (4.97±0.98 v. 10.18±4.76 ng/ml, p<0.05). HIV-1 RNA was positively correlated with urinary MMP-9 concentration in HIV (+) (r=0.29, p<0.05). Moreover urinary MMP-9 concentration was positively correlated with urinary BMP-7 concentration (r=0.34, p = 0.005) and negatively correlated with creatine clearance (r=-0.32, p<0.05) in studied population.

Conclusion: Increased urinary MMP-9 concentration is associated with plasma HIV viral load and could be a new biomarker of subclinical renal lesion. MMP-9 augment together with BMP-7 increase may be explained by compensatory protection mechanism against renal damage.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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